Development of 19F-NMR chemical shift detection of DNA B–Z equilibrium using 19F-NMR

Abstract

Various DNA conformational changes are in correlation with biological events. In particular, DNA B–Z equilibrium showed a high correlation with translation and transcription. In this study, we developed a DNA probe containing 5-trifluoromethylcytidine or 5-trifluoromethylthymidine to detect DNA B–Z equilibrium using ¹⁹F-NMR. Its probe enabled the quantitative detection of B-, Z-, and ss-DNA based on ¹⁹F-NMR chemical shift change.