Issue 42, 2017

Driving and photo-regulation of myosin–actin motors at molecular and macroscopic levels by photo-responsive high energy molecules

Abstract

We employed an azobenzene based non-nucleoside triphosphate, AzoTP, in a myosin–actin motile system and demonstrated its efficiency as an energy molecule to drive and photo-regulate the myosin–actin motile function at the macroscopic level along with an in vitro motility assay. The AzoTP in its trans state induced shortening of a glycerinated muscle fibre whilst a cis isomer had no significant effect. Direct photoirradiation of a cis-AzoTP infused muscle fibre induced shortening triggered by a locally photo-generated trans-AzoTP in the muscle fibre. Furthermore, we designed and synthesized three new derivatives of AzoTPs that served as substrates for myosin by driving and photo-regulating the myosin–actin motile function at the molecular as well as the macroscopic level with varied efficiencies.

Graphical abstract: Driving and photo-regulation of myosin–actin motors at molecular and macroscopic levels by photo-responsive high energy molecules

Supplementary files

Article information

Article type
Paper
Submitted
26 May 2017
Accepted
03 Sep 2017
First published
13 Sep 2017

Org. Biomol. Chem., 2017,15, 8894-8903

Driving and photo-regulation of myosin–actin motors at molecular and macroscopic levels by photo-responsive high energy molecules

H. M. Menezes, Md. J. Islam, M. Takahashi and N. Tamaoki, Org. Biomol. Chem., 2017, 15, 8894 DOI: 10.1039/C7OB01293D

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