Issue 42, 2017

A cryptophane-based “turn-on” 129Xe NMR biosensor for monitoring calmodulin

Abstract

We present the first cryptophane-based “turn-on” 129Xe NMR biosensor, employing a peptide-functionalized cryptophane to monitor the activation of calmodulin (CaM) protein in solution. In the absence of CaM binding, interaction between the peptide and cryptophane completely suppresses the hyperpolarized 129Xe-cryptophane NMR signal. Biosensor binding to Ca2+-activated CaM produces the expected 129Xe-cryptophane NMR signal.

Graphical abstract: A cryptophane-based “turn-on” 129Xe NMR biosensor for monitoring calmodulin

Supplementary files

Article information

Article type
Communication
Submitted
24 Sep 2017
Accepted
16 Oct 2017
First published
16 Oct 2017

Org. Biomol. Chem., 2017,15, 8883-8887

A cryptophane-based “turn-on” 129Xe NMR biosensor for monitoring calmodulin

B. A. Riggle, M. L. Greenberg, Y. Wang, R. F. Wissner, S. D. Zemerov, E. J. Petersson and I. J. Dmochowski, Org. Biomol. Chem., 2017, 15, 8883 DOI: 10.1039/C7OB02391J

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