A hydrophilicity-based fluorescent strategy to differentiate cysteine/homocysteine over glutathione both in vivo and in vitro

Xiaoman Peng; Hua Yuan; Jian Xu; Fengxian Lu; Liangqian Wang; Xudong Guo; Shuangqing Wang; Shayu Li; Yi Li; Guoqiang Yang

doi:10.1039/C6RA27074C

A hydrophilicity-based fluorescent strategy to differentiate cysteine/homocysteine over glutathione both in vivo and in vitro†

Xiaoman Peng,‡^a Hua Yuan,‡^a Jian Xu,^a Fengxian Lu,^a Liangqian Wang,^a Xudong Guo,^a Shuangqing Wang,^a Shayu Li,*^a Yi Li*^b and Guoqiang Yang

*^a

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* Corresponding authors

^a Beijing National Laboratory for Molecular Sciences, Key Laboratory of Photochemistry, Institute of Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100190, China
E-mail: gqyang@iccas.ac.cn, shayuli@iccas.ac.cn
Fax: +86 10 82617315
Tel: +86 10 82617263

^b Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
E-mail: yili@mail.ipc.ac.cn

Abstract

An easily-prepared probe/nanogel composite indicator HTBNM/PU was presented, which showed selective fluorescence responses to cysteine/homocysteine over glutathione both in vivo and in vitro based on the different hydrophilicity of these biothiols. In principle, this approach could endow any lipophilic thiol indicator with selectivity for sulfur-containing amino acids.

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A hydrophilicity-based fluorescent strategy to differentiate cysteine/homocysteine over glutathione both in vivo and in vitro†

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A hydrophilicity-based fluorescent strategy to differentiate cysteine/homocysteine over glutathione both in vivo and in vitro

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