The effects of dissociated rods and rod-surfaced microspheres on bone mesenchymal stem cellular viability

Abstract

Material properties and cellular behaviours seemed to be coupled, implying the existence of reciprocities between cells and materials. Understanding cellular behaviours and material structure is indispensable for materials synthesis and design but has mostly focused on shape, neglecting the status of the sub-structure. Hence we utilized a similar route to regulate the different morphology of HAp particles and then investigated reciprocities between particles and bone marrow mesenchymal stem cells (BMSCs). Based on our cell experiment, we revealed the side effects of dissociated micro-rods on cellular behaviours. Despite the facts that both particles showed modestly cytotoxicity, the multi-structure HAp sphere could positively-regulate the proliferation, viability and differentiation of bone marrow mesenchymal stem cells (BMSCs) which indicated that the dissociated micro-rods may deteriorate the biological properties in vitro.