A cancer cell-specific two-photon fluorescent probe for imaging hydrogen sulfide in living cells

Abstract

Hydrogen sulfide (H₂S) could induce the proliferation of cancer cells in a concentration-dependent manner, and has close relation with the tumor growth. Monitoring the H₂S level in real-time is of great important for understanding its roles in the cancer cell proliferation and the diagnosis of the tumor. Herein, a novel cancer cell-specific two-photon fluorescent probe BN-H₂S for detecting H₂S in cancer cells was designed and synthesized. Biotin was selected as the cancer cell-specific group and the azide group was employed as the response site for H₂S. When BN-H₂S responded to H₂S, a turn-on fluorescence at 544 nm was observed clearly. BN-H₂S exhibited high selectivity for H₂S over other relative species. Under the guidance of the biotin group, BN-H₂S can be successfully used for the two-photon imaging of H₂S in cancer cells, while BN-H₂S showed relatively weak response for H₂S in normal cells. We expect that this design concept can be further developed for selectively detecting other biomolecules in living cancer cells.