Issue 75, 2017, Issue in Progress

The screening of metal ion inhibitors for glucose oxidase based on the peroxidase-like activity of nano-Fe3O4

Abstract

In this study, a colorimetric method is proposed based on the peroxidase-like activity of Fe3O4 magnetic nanoparticles for screening metal ion inhibitors for glucose oxidase activity. First, the glucose oxidase was typically used as a specific enzyme to catalyze the oxidation of β-D-glucose resulting in the generation of hydrogen peroxide. Next, having an inherent peroxidase-like activity, Fe3O4 magnetic nanoparticles were adopted as the catalyst. Then, the generated H2O2 was capable of participating in the oxidation of 3,3′,5,5′-tetramethylbenzidine to yield a blue colored product. Based on the above results, an in vitro screen model of metal ion inhibitors of glucose oxidase was thus established. Metal ions including Ca2+, Pb2+, Mn2+, Ag+, Al3+, Cu2+, Mg2+ and Zn2+ have been tested. Herein, towards the glucose oxidase activity, Ca2+, Pb2+, Mg2+ and Mn2+ showed no effect while Al3+ and Zn2+ displayed a slight activation, while of Ag+ and Cu2+ expressed a strong inhibition. The further detection of Ag+ and Cu2+ manifested that their IC50 were 0.662 μmol L−1 and 12.619 μmol L−1, respectively. The entire detection process could be accomplished within 15 min. This assay is economical, time-saving and highly-effective with definitely significant reference for the screening of metal ions as glucose oxidase inhibitors.

Graphical abstract: The screening of metal ion inhibitors for glucose oxidase based on the peroxidase-like activity of nano-Fe3O4

Article information

Article type
Paper
Submitted
26 Jun 2017
Accepted
13 Sep 2017
First published
09 Oct 2017
This article is Open Access
Creative Commons BY license

RSC Adv., 2017,7, 47309-47315

The screening of metal ion inhibitors for glucose oxidase based on the peroxidase-like activity of nano-Fe3O4

Y. Wu, L. Chu, W. Liu, L. Jiang, X. Chen, Y. Wang and Y. Zhao, RSC Adv., 2017, 7, 47309 DOI: 10.1039/C7RA07081K

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements