Molecular recognition of pyruvic acid and folic acid in whole blood

Abstract

Pyruvic and folic acids need to be monitored in both young and old persons, not only to assess the ageing state, but also to diagnose illnesses at an early stage, e.g., diabetes. Therefore, there is a need to develop highly reliable tools and methods of analysis for their determination in biological fluids (e.g., whole blood samples). Three new stochastic microsensors based on the physical immobilization of α, β, and γ-cyclodextrins (CD) in the paste of a TiO₂Pt–graphene nanocomposite (TiO₂Pt/rGO) were prepared; the novelty of the stochastic sensors used for the assay of the biomolecules from biological fluids is given by the utilization of TiO₂Pt–graphene nanocomposite as the matrix; this matrix supported the CDs' channels used for stochastic sensing, better than other materials. The proposed microsensors are able to recognize and determine pyruvate and folate in whole blood samples at very low concentrations. The recovery tests performed for the assay of pyruvate and folate in whole blood samples proved that they can be assayed with a recovery higher than 98.00% and an RSD value lower than 1.00%.