Issue 8, 2017

Structural-functional analysis of engineered protein-nanoparticle assemblies using graphene microelectrodes

Abstract

The characterization of protein-nanoparticle assemblies in solution remains a challenge. We demonstrate a technique based on a graphene microelectrode for structural-functional analysis of model systems composed of nanoparticles enclosed in open-pore and closed-pore ferritin molecules. The method readily resolves the difference in accessibility of the enclosed nanoparticle for charge transfer and offers the prospect for quantitative analysis of pore-mediated transport, while shedding light on the spatial orientation of the protein subunits on the nanoparticle surface, faster and with higher sensitivity than conventional catalysis methods.

Graphical abstract: Structural-functional analysis of engineered protein-nanoparticle assemblies using graphene microelectrodes

Supplementary files

Article information

Article type
Edge Article
Submitted
07 Apr 2017
Accepted
12 Jun 2017
First published
13 Jun 2017
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2017,8, 5329-5334

Structural-functional analysis of engineered protein-nanoparticle assemblies using graphene microelectrodes

J. Ping, Katherine W. Pulsipher, R. Vishnubhotla, J. A. Villegas, T. L. Hicks, S. Honig, J. G. Saven, I. J. Dmochowski and A. T. C. Johnson, Chem. Sci., 2017, 8, 5329 DOI: 10.1039/C7SC01565H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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