Lactoferrin-modified nanoparticles loaded with potent antioxidant Mn-porphyrins exhibit enhanced antioxidative activity in vitro intranasal brain delivery model

Abstract

In this study, for efficient intranasal brain delivery, we have prepared lactoferrin (Lf)-modified nanoparticles loaded with an amphiphilic Mn-porphyrin derivative, MndMImP₃P (MnP) (Lf-NP-MnP). MndMImP₃P was loaded into the nanoparticles with good loading efficiency. The stability of the resulting Lf-NP-MnP was sufficient for intranasal brain delivery. Lf on the surface of Lf-NP-MnP was significantly recognized by the Lf receptor, which leads to enhanced cellular uptake of MnP and efficient transcytosis. Furthermore, Lf-NP-MnP exhibited antioxidative activity in vitro experiment using a transwell assay as a model of intranasal brain delivery. Our result in this study is one step forward for efficient brain delivery of Mn-porphyrin derivatives to cure neurodegenerative diseases.