Issue 12, 2017

Cell-inspired biointerfaces constructed from patterned smart hydrogels for immunoassays in whole blood

Abstract

Immunoassays have shown great advances in the fields of biomedical diagnosis. However, successful immunoassays in blood plasma or whole blood based on the designed biointerfaces are still rare. Here, a newly cell-inspired biointerface for immunoassays in blood is demonstrated. Inspired by the high resistance to protein and cell adhesion and extraordinary biological recognition of stem cells, the biointerfaces are constructed by patterning smart hydrogels (PNIPAAm-co-PNaAc) on hydrophilic layers (PEG), followed by immobilization of antibodies on the patterned hydrogels. The hierarchical biointerfaces are hydrophilic to resist blood plasma and blood cell adhesion, but exhibit high affinity to the target antigens. As a result, successful immunoassays in blood are achieved. In addition, the detection signal is further enhanced by the manipulation of the phase transition of the smart hydrogels with temperature, and the sensitivity is higher than that of the widely-used poly(acrylic acid)/(polyacrylate) platform. The biointerface is versatile and effective in antibody–antigen recognition, which offers a potential new approach for developing highly sensitive immunoassays in blood.

Graphical abstract: Cell-inspired biointerfaces constructed from patterned smart hydrogels for immunoassays in whole blood

Supplementary files

Article information

Article type
Paper
Submitted
29 Dec 2016
Accepted
26 Feb 2017
First published
27 Feb 2017

J. Mater. Chem. B, 2017,5, 2315-2321

Cell-inspired biointerfaces constructed from patterned smart hydrogels for immunoassays in whole blood

C. Zhao, J. Hou, R. Chen, Z. Xin, H. Shi, S. Wong, J. Yin and Q. Shi, J. Mater. Chem. B, 2017, 5, 2315 DOI: 10.1039/C6TB03385G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements