Issue 1, 2018

Towards the electrochemical diagnostic of influenza virus: development of a graphene–Au hybrid nanocomposite modified influenza virus biosensor based on neuraminidase activity

Abstract

An effective electrochemical influenza A biosensor based on a graphene–gold (Au) hybrid nanocomposite modified Au-screen printed electrode has been developed. The working principle of the developed biosensor relies on the measurement of neuraminidase (N) activity. After the optimization of experimental parameters like the effect of bovine serum albumin addition and immobilization times of fetuin A and PNA lectin, the analytical characteristics of the influenza A biosensor were investigated. As a result, a linear range between 10−8 U mL−1 and 10−1 U mL−1 was found with a relative standard deviation value of 3.23% (for 10−5 U mL−1 of N, n:3) and a limit of detection value of 10−8 U mL−1 N. The developed biosensor was applied for real influenza virus A (H9N2) detection and very successful results were obtained.

Graphical abstract: Towards the electrochemical diagnostic of influenza virus: development of a graphene–Au hybrid nanocomposite modified influenza virus biosensor based on neuraminidase activity

Supplementary files

Article information

Article type
Paper
Submitted
15 Sep 2017
Accepted
25 Oct 2017
First published
26 Oct 2017

Analyst, 2018,143, 150-156

Towards the electrochemical diagnostic of influenza virus: development of a graphene–Au hybrid nanocomposite modified influenza virus biosensor based on neuraminidase activity

Ü. Anik, Y. Tepeli, M. Sayhi, J. Nsiri and M. F. Diouani, Analyst, 2018, 143, 150 DOI: 10.1039/C7AN01537B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements