Issue 3, 2018

Label-free detection of fibrinogen based on the fibrinogen-enhanced peroxidase activity of a fibrinogen–hemin composite

Abstract

A simple, label-free colorimetric method for the determination of fibrinogen (Fib) in plasma is presented. In this work, it was observed that Fib interacted with hemin to form a hemin–Fib composite. Because Fib prevented hemin from the formation of m-oxo-dimers, the hemin–Fib composite possesses excellent peroxidase-like activity. Importantly, the peroxidase-like activity of Fib–hemin increased with the increase in the Fib. This allows us to utilize the H2O2–ABTS colorimetric system for the quantitative analysis of Fib. This optimized method provided a linear determination range of 2.0–100 pM with a correlation of 0.9975. The limit of detection for Fib was experimentally determined to be 0.7 pM based on a signal-to-noise ratio (S/N) of 3. This novel approach provides a rapid, sensitive, cost efficient and robust bioassay for detection of Fib in pathology and clinical applications.

Graphical abstract: Label-free detection of fibrinogen based on the fibrinogen-enhanced peroxidase activity of a fibrinogen–hemin composite

Supplementary files

Article information

Article type
Paper
Submitted
08 Oct 2017
Accepted
16 Dec 2017
First published
19 Dec 2017

Analyst, 2018,143, 725-730

Label-free detection of fibrinogen based on the fibrinogen-enhanced peroxidase activity of a fibrinogen–hemin composite

T. Hou, Y. Zhang, T. Wu, M. Wang, Y. Zhang, R. Li, L. Wang, Q. Xue and S. Wang, Analyst, 2018, 143, 725 DOI: 10.1039/C7AN01661A

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