A PCR-free technology to detect and quantify microRNAs directly from human plasma

Antonio Marín-Romero; Agustín Robles-Remacho; Mavys Tabraue-Chávez; Bárbara López-Longarela; Rosario M. Sánchez-Martín; Juan J. Guardia-Monteagudo; Mario A. Fara; Francisco J. López-Delgado; Salvatore Pernagallo; Juan J. Díaz-Mochón

doi:10.1039/C8AN01397G

A PCR-free technology to detect and quantify microRNAs directly from human plasma†

Antonio Marín-Romero,^abc Agustín Robles-Remacho,^bc Mavys Tabraue-Chávez,^a Bárbara López-Longarela,^a Rosario M. Sánchez-Martín,

^bc Juan J. Guardia-Monteagudo,^a Mario A. Fara,^a Francisco J. López-Delgado,

^a Salvatore Pernagallo

*^a and Juan J. Díaz-Mochón

*^bc

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* Corresponding authors

^a DestiNA Genomica S.L. Parque Tecnológico Ciencias de la Salud (PTS), Avenida de la Innovación 1, Edificio BIC, Armilla, Granada 18100, Spain
E-mail: salvatore@destinagenomics.com

^b GENYO. Centre for Genomics and Oncological Research: Pfizer/University of Granada/Andalusian Regional Government, PTS Granada - Avenida de la Ilustración, 114 - 18016 GRANADA, Spain
E-mail: juanjose.diaz@genyo.es

^c Universidad de Granada. Facultad de Farmacia. Departamento de Quimica Farmacéutica y Orgánica, Campus Cartuja s/n, 18071 Granada, Spain

Abstract

A novel sensitive, specific and rapid method for the detection and quantification of microRNAs without requiring extraction from their biological sources is now available using a novel chemical based, PCR-free technology for nucleic acid testing. In this study, we both demonstrate how this method can be used to profile miR-451a, an important miRNA in erythropoiesis, and compare with the gold standard RT-qPCR.

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A PCR-free technology to detect and quantify microRNAs directly from human plasma†

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A PCR-free technology to detect and quantify microRNAs directly from human plasma

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