Enhanced peroxidase-like activity of Mo-doped ceria nanoparticles for sensitive colorimetric detection of glucose

Abstract

The development of highly efficient nanomaterial-based mimetic enzymes is much needed to replace natural enzymes due to their low cost, high stability and efficient catalytic ability. Herein, multiple Mo-doped CeO₂ nanoparticles (Mo/CeO₂ NPs) with different molar ratios of Mo were synthesized using a facile microwave-assisted method. For characterizing the as-prepared Mo/CeO₂ NPs, XRD, TEM, HRTEM, BET and XPS have been performed systematically. The peroxidase-like activity of Mo/CeO₂ NPs was investigated, and they exhibited enhanced peroxidase-like activity in comparison to that of pure CeO₂ NPs, the best peroxidase-like activity was obtained for the Mo/CeO₂ NPs when the molar ratio of Mo was 9%. On the basis of the high activity of Mo/CeO₂ NPs, the reaction provides a simple, sensitive and selective method for colorimetric detection of glucose with the detection limit of 4 × 10⁻⁷ mol L⁻¹ and in a dynamic range from 5 × 10⁻⁷ to 5 × 10⁻⁵ mol L⁻¹. This work will pave a way for high throughput synthesis of other ultra-small doped metal oxide nanoparticles, which have bright prospects in biomedical analysis.