Determination of Cu2+ and biothiols by novel red fluorescent hybrid nanoparticles

Abstract

Red fluorescent hybrid nanoparticles (NPG@PFBD) have been designed and synthesized successfully. The NPG@PFBD nanoparticles are composed of a fluorescent semiconducting polymer (PFBD) and n-propyl gallate (NPG). Because of the coordination between the gallate and Cu²⁺, Cu²⁺ could attach to the hybrid nanoparticles and quench their fluorescence through photoinduced electron transfer (PET). In addition, in the presence of biothiols, such as glutathione (GSH), Cu²⁺ would bind to the thiol. The PET between the hybrid nanoparticles and Cu²⁺ was weakened, and the fluorescence signal of the nanoparticles was recovered. A facile detection method for Cu²⁺ and biothiols was therefore developed. The detection limit for Cu²⁺ and GSH was 40 nM and 90 nM, respectively. The proposed fluorescent hybrid nanoparticles have great potential application for Cu²⁺ and GSH detection in related biological fields.