Issue 37, 2018

Matrix effect of five kinds of meat on colloidal gold immunochromatographic assay for sulfamethazine detection

Abstract

A colloidal gold immunochromatographic assay (CG-ICA) was developed to detect sulfamethazine (SM2) residues in five kinds of meat: namely, chicken, pork, shrimp, beef, and fish. The limit of detection (LOD) of CG-ICA for SM2 detection was 0.104 ng mL−1 within 20 min. CG-ICA exhibited a wide linear range of 0.1 ng mL−1 to 96 ng mL−1 with a reliable correlation coefficient (R2 = 0.9938). The average recoveries of CG-ICA for SM2 detection in chicken, pork, shrimp, beef, and fish were 98.1% ± 4.6%, 86.1% ± 0.5%, 86.2% ± 4.9%, 71.7% ± 1.7%, and 79.2% ± 2.5%, respectively. The matrix effect of the five kinds of meat on CG-ICA was also assessed. The standard curves of the five matrices did not coincide with the standard curve of phosphate buffer saline, indicating that a matrix effect had occurred in CG-ICA for SM2 detection in the five matrices. The strongest matrix effect was elicited by beef, followed by fish, pork, and shrimp, whereas the weakest matrix effect was produced by chicken.

Graphical abstract: Matrix effect of five kinds of meat on colloidal gold immunochromatographic assay for sulfamethazine detection

Supplementary files

Article information

Article type
Paper
Submitted
12 Jun 2018
Accepted
02 Aug 2018
First published
03 Aug 2018

Anal. Methods, 2018,10, 4505-4510

Matrix effect of five kinds of meat on colloidal gold immunochromatographic assay for sulfamethazine detection

N. Liu, K. Xing, C. Wang, G. Zhang, M. Yuan, D. Li, W. Lai and J. Peng, Anal. Methods, 2018, 10, 4505 DOI: 10.1039/C8AY01322E

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