Issue 6, 2018
From the journal:

Chemical Communications

Amplified detection of genome-containing biological targets using terminal deoxynucleotidyl transferase-assisted rolling circle amplification†

Yi-Chen Du,a   Yan-Jun Zhu,a   Xiao-Yu Lia  and  De-Ming Kong ORCID logo *ab  

* Corresponding authors

a State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Nankai University, Tianjin, P. R. China
E-mail: kongdem@nankai.edu.cn

b Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, P. R. China

Abstract

We proposed a terminal deoxynucleotidyl transferase (TdT)-assisted rolling circle amplification (RCA) strategy for the amplified detection of genome-containing biological targets. The synergetic signal amplification of DNase I-mediated genomic DNA fragmentation and subsequent RCA allow the sensing platform to have extraordinarily high sensitivity.

Graphical abstract: Amplified detection of genome-containing biological targets using terminal deoxynucleotidyl transferase-assisted rolling circle amplification

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C7CC09337C
Article type
Communication
Submitted
05 Dec 2017
Accepted
19 Dec 2017
First published
19 Dec 2017

Chem. Commun., 2018,54, 682-685

Permissions

Request permissions

Amplified detection of genome-containing biological targets using terminal deoxynucleotidyl transferase-assisted rolling circle amplification

Y. Du, Y. Zhu, X. Li and D. Kong, Chem. Commun., 2018, 54, 682 DOI: 10.1039/C7CC09337C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements