Issue 13, 2018

Orthogonal supramolecular protein assembly on patterned bifunctional surfaces

Abstract

We report successful and selective dual protein assembly on patterned bifunctional βCD-Ni(II)NTA surfaces, using red fluorescent protein variants with hexahistidine-tags and teal fluorescent protein variants conjugated with a peptide containing three adamantyl groups. We show that dual protein patterns can only be assembled, when opposing supramolecular interactions have been optimized and nonspecific interactions have been sufficiently suppressed.

Graphical abstract: Orthogonal supramolecular protein assembly on patterned bifunctional surfaces

Supplementary files

Article information

Article type
Communication
Submitted
03 Jan 2018
Accepted
19 Jan 2018
First published
19 Jan 2018

Chem. Commun., 2018,54, 1615-1618

Orthogonal supramolecular protein assembly on patterned bifunctional surfaces

D. Wasserberg, J. Cabanas-Danés, V. Subramaniam, J. Huskens and P. Jonkheijm, Chem. Commun., 2018, 54, 1615 DOI: 10.1039/C7CC09808A

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