Issue 24, 2018
From the journal:

Chemical Communications

Genetic incorporation of l-dihydroxyphenylalanine (DOPA) biosynthesized by a tyrosine phenol-lyase†

Sanggil Kim,a   Bong Hyun Sung,b   Sun Chang Kimc  and  Hyun Soo Lee ORCID logo *a  

* Corresponding authors

a Department of Chemistry, Sogang University, Seoul 121-742, Republic of Korea
E-mail: hslee76@sogang.ac.kr

b Bioenergy and Biochemical Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea

c Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea

Abstract

L-Dihydroxyphenylalanine (DOPA) was biosynthesized by a tyrosine-phenol lyase from catechol, pyruvate, and ammonia in Escherichia coli, and the biosynthesized amino acid was directly incorporated into proteins. Three biochemical experiments with mutant proteins containing DOPA confirmed the genetic incorporation of biosynthesized DOPA, and revealed its potential for various biochemical applications.

Graphical abstract: Genetic incorporation of l-dihydroxyphenylalanine (DOPA) biosynthesized by a tyrosine phenol-lyase

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Article information

DOI
https://doi.org/10.1039/C8CC00281A
Article type
Communication
Submitted
12 Jan 2018
Accepted
12 Feb 2018
First published
26 Feb 2018

Chem. Commun., 2018,54, 3002-3005

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Genetic incorporation of L-dihydroxyphenylalanine (DOPA) biosynthesized by a tyrosine phenol-lyase

S. Kim, B. H. Sung, S. C. Kim and H. S. Lee, Chem. Commun., 2018, 54, 3002 DOI: 10.1039/C8CC00281A

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