Issue 36, 2018

Anti-Stokes fluorescence microscopy using direct and indirect dark state formation

Abstract

Measurements on biological samples are often hampered by auto-fluorescence from inherent compounds in tissue or cells, limiting the achievable contrast. Both the signal of interest and the auto-fluorescence are usually detected on the Stokes side of the excitation laser. In this communication, we present two new microscopy modalities, based on the emission of a red-emitting DNA-stabilized silver nanocluster (DNA-AgNC). Its bright fluorescence can be generated on the anti-Stokes side of the readout laser, allowing easy spectral separation of the signal of interest from the Stokes side auto-fluorescence.

Graphical abstract: Anti-Stokes fluorescence microscopy using direct and indirect dark state formation

Supplementary files

Article information

Article type
Communication
Submitted
23 Feb 2018
Accepted
05 Apr 2018
First published
17 Apr 2018
This article is Open Access
Creative Commons BY license

Chem. Commun., 2018,54, 4569-4572

Anti-Stokes fluorescence microscopy using direct and indirect dark state formation

S. Krause, M. R. Carro-Temboury, C. Cerretani and T. Vosch, Chem. Commun., 2018, 54, 4569 DOI: 10.1039/C8CC01521J

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