Issue 52, 2018

Endogenous exosome labelling with an amphiphilic NIR-fluorescent probe

Abstract

The recognition of the biological, diagnostic and medical importance of exosomes has given rise to an urgent need for efficient labelling of these extracellular vesicles in ways that do not alter their inherent characteristics. We report for the first time an endogenous method to NIR-fluorescent labelled exosomes using an amphiphilic probe without the need for immunolabelling or synthetic or chromatographic manipulation of exosomes. Comparative analyses of labelled and unlabelled exosomes with NTA, AFM, flow cytometry and immunoblot analysis all show a high degree of similarity. Spectroscopic analysis and fluorescence imaging confirmed the ability to visualise purified NIR-exosomes.

Graphical abstract: Endogenous exosome labelling with an amphiphilic NIR-fluorescent probe

Supplementary files

Article information

Article type
Communication
Submitted
16 Mar 2018
Accepted
27 May 2018
First published
14 Jun 2018

Chem. Commun., 2018,54, 7219-7222

Endogenous exosome labelling with an amphiphilic NIR-fluorescent probe

M. P. Monopoli, A. Zendrini, D. Wu, S. Cheung, G. Sampedro, B. Ffrench, J. Nolan, O. Piskareva, R. L. Stalings, S. Ducoli, P. Bergese and D. F. O’Shea, Chem. Commun., 2018, 54, 7219 DOI: 10.1039/C8CC02135J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements