Quantification of methylation efficiency at a specific N6-methyladenosine position in rRNA by using BNA probes

Abstract

We found that insertion of artificial nucleic acid analogs, such as bridged nucleic acid (BNA), into DNA probes increases the difference in melting temperature between N⁶-methyladenosine (m⁶A)-containing RNA and unmethylated RNA. By applying this principle, we quantified methylation efficiency at m⁶A sites in E. coli 23S rRNA with high accuracy.