Issue 68, 2018
From the journal:

Chemical Communications

Design and synthesis of ortho-phthalaldehyde phosphoramidite for single-step, rapid, efficient and chemoselective coupling of DNA with proteins under physiological conditions†

Yanli Ma,a   Zhehao Lv,a   Tingyu Li,a   Tian Tian,a   Lianyu Lu,a   Wenli Liu,a   Zhi Zhua  and  Chaoyong Yang ORCID logo *ab  

* Corresponding authors

a State Key Laboratory of Physical Chemistry of Solid Surfaces, MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Collaborative Innovation Center of Chemistry for Energy Materials, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
E-mail: cyyang@xmu.edu.cn

b Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China

Abstract

ortho-Phthalaldehyde (OPA) phosphoramidite with high reaction activity was designed and synthesized for labelling oligodeoxynucleotides (DNA). The DNA modified with OPA (OPA-DNA) can covalently couple with native proteins rapidly and efficiently via a condensation reaction with the formation of phthalimidines, which provides a highly efficient method for bioconjugation of DNA and native proteins under physiological conditions.

Graphical abstract: Design and synthesis of ortho-phthalaldehyde phosphoramidite for single-step, rapid, efficient and chemoselective coupling of DNA with proteins under physiological conditions

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Article information

DOI
https://doi.org/10.1039/C8CC05037F
Article type
Communication
Submitted
26 Jun 2018
Accepted
30 Jul 2018
First published
30 Jul 2018

Chem. Commun., 2018,54, 9434-9437

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Design and synthesis of ortho-phthalaldehyde phosphoramidite for single-step, rapid, efficient and chemoselective coupling of DNA with proteins under physiological conditions

Y. Ma, Z. Lv, T. Li, T. Tian, L. Lu, W. Liu, Z. Zhu and C. Yang, Chem. Commun., 2018, 54, 9434 DOI: 10.1039/C8CC05037F

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