Low catalytic activity of the Cu(ii)-binding motif (Xxx-Zzz-His; ATCUN) in reactive oxygen species production and inhibition by the Cu(i)-chelator BCS

Abstract

The catalytic redox activity of Cu(II) bound to the motif NH₂–Xxx-Zzz-His (ATCUN) with ascorbate and H₂O₂/O₂ is very low and can be stopped via Cu(I)-chelation. This impacts its application as an artificial Cu–enzyme to degrade biomolecules via production of reactive oxygen species in a Cu(I)-chelator rich environment like the cytosol.