Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes

Yoshihide Tokunou; Punthira Chinotaikul; Shingo Hattori; Thomas A. Clarke; Liang Shi; Kazuhito Hashimoto; Kazuyuki Ishii; Akihiro Okamoto

doi:10.1039/C8CC06309E

Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes†‡

Yoshihide Tokunou,

^a Punthira Chinotaikul,^a Shingo Hattori,^b Thomas A. Clarke,^c Liang Shi,^d Kazuhito Hashimoto,^e Kazuyuki Ishii

^b and Akihiro Okamoto*^e

Author affiliations

* Corresponding authors

^a Department of Applied Chemistry, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan

^b Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8605, Japan

^c Centre for Molecular and Structural Biochemistry, School of Biological Sciences and School of Chemistry, University of East Anglia, Norwich NR4 7TJ, UK

^d Department of Biological Sciences, School of Environmental Studies, The China University of Geosciences, Wuhan, Hubei 430074, P. R. China

^e International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
E-mail: okamoto.akihiro@nims.go.jp

Abstract

We established whole-cell circular dichroism difference spectroscopy to identify the inter-heme interaction in deca-heme cytochrome protein MtrC in whole cell. Our data showed that the heme alignment of reduced MtrC in whole cell is distinct from that in purified one, suggesting the in vivo specific electron transport kinetics.

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DOI: https://doi.org/10.1039/C8CC06309E
Article type: Communication
Submitted: 02 Aug 2018
Accepted: 12 Sep 2018
First published: 07 Nov 2018
This article is Open Access

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Chem. Commun., 2018,54, 13933-13936

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Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes

Y. Tokunou, P. Chinotaikul, S. Hattori, T. A. Clarke, L. Shi, K. Hashimoto, K. Ishii and A. Okamoto, Chem. Commun., 2018, 54, 13933 DOI: 10.1039/C8CC06309E

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Chemical Communications

Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes†‡

Abstract

Supplementary files

Article information

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Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes

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