Issue 95, 2018

A fluorescence assay for the detection of hydrogen peroxide and hydroxyl radicals generated by metallonucleases

Abstract

Metal complexes can initiate DNA cleavage by the formation of reactive oxygen species (ROS). A conventional assay to probe for ROS is to add quenchers in a gel electrophoresis experiment. As we show here, such an assay is neither selective nor reliable. Instead, we suggest the use of simple fluorogens, as tested here with several metallonucleases for the detection of H2O2 and HO˙.

Graphical abstract: A fluorescence assay for the detection of hydrogen peroxide and hydroxyl radicals generated by metallonucleases

Supplementary files

Article information

Article type
Communication
Submitted
29 Aug 2018
Accepted
06 Nov 2018
First published
06 Nov 2018

Chem. Commun., 2018,54, 13411-13414

A fluorescence assay for the detection of hydrogen peroxide and hydroxyl radicals generated by metallonucleases

S. Leichnitz, J. Heinrich and N. Kulak, Chem. Commun., 2018, 54, 13411 DOI: 10.1039/C8CC06996D

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