A ratiometric fluorescent probe for peroxynitrite prepared by de novo synthesis and its application in assessing the mitochondrial oxidative stress status in cells and in vivo

Abstract

A ratiometric fluorescent probe Cy-NEt₂ for detecting ONOO⁻ is designed and prepared by de novo synthesis, which is a reliable, cheap and flexible route. The probe is applied for monitoring the level of mitochondrial ONOO⁻ and assessing the lipopolysaccharide (LPS)-induced mitochondrial oxidative stress status in living cells and in vivo successfully.