Issue 96, 2018
From the journal:

Chemical Communications

High-depth fluorescence imaging using a two-photon FRET system for mitochondrial pH in live cells and tissues†

Min Jung Chang,a   Kyutae Kim,b   Kyun Seob Park,b   Ji Su Kang,c   Chang Su Lim,*c   Hwan Myung Kim, ORCID logo *c   Chulhun Kang*b  and  Min Hee Lee ORCID logo *a  

* Corresponding authors

a Department of Chemistry, Sookmyung Women's University, Seoul, Korea
E-mail: minheelee@sookmyung.ac.kr

b The School of East-West Medical Science, Kyung Hee University, Yongin, Korea
E-mail: kangch@khu.ac.kr

c Department of Chemistry, Ajou University, Suwon, Korea
E-mail: cslim8112@gmail.com, kimhm@ajou.ac.kr

Abstract

We developed a fluorescent pH probe (1) capable of two-photon excitation and far-visible-emission based on FRET, composed of naphthalimide–piperazine–rhodamine. It exhibited a pH-dependent reversible and fast ratiometric fluorescence change in the rhodamine emission. Probe 1 was applied to image the pH perturbations of mitochondria in living cells and tissues.

Graphical abstract: High-depth fluorescence imaging using a two-photon FRET system for mitochondrial pH in live cells and tissues

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Article information

DOI
https://doi.org/10.1039/C8CC07934J
Article type
Communication
Submitted
04 Oct 2018
Accepted
06 Nov 2018
First published
07 Nov 2018

Chem. Commun., 2018,54, 13531-13534

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High-depth fluorescence imaging using a two-photon FRET system for mitochondrial pH in live cells and tissues

M. J. Chang, K. Kim, K. S. Park, J. S. Kang, C. S. Lim, H. M. Kim, C. Kang and M. H. Lee, Chem. Commun., 2018, 54, 13531 DOI: 10.1039/C8CC07934J

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