The N-terminal 14-mer model peptide of human Ctr1 can collect Cu(II) from albumin. Implications for copper uptake by Ctr1

Ewelina Stefaniak; Dawid Płonka; Simon C. Drew; Karolina Bossak-Ahmad; Kathryn L. Haas; M. Jake Pushie; Peter Faller; Nina E. Wezynfeld; Wojciech Bal

doi:10.1039/C8MT00274F

The N-terminal 14-mer model peptide of human Ctr1 can collect Cu(ii) from albumin. Implications for copper uptake by Ctr1†

Ewelina Stefaniak,^a Dawid Płonka,^a Simon C. Drew,

^ab Karolina Bossak-Ahmad,

^a Kathryn L. Haas,

^c M. Jake Pushie,

^d Peter Faller,

^e Nina E. Wezynfeld

*^ae and Wojciech Bal

*^a

Author affiliations

* Corresponding authors

^a Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland
E-mail: nina@ibb.waw.pl, wbal@ibb.waw.pl

^b Department of Medicine, Royal Melbourne Hospital, University of Melbourne, Melbourne, VIC 3010, Australia

^c Department of Chemistry and Physics, Saint Mary's College, Notre Dame, Indiana 46556, USA

^d Department of Surgery, Division of Neurosurgery, College of Medicine, University of Saskatchewan, 107 Wiggins Road, Saskatoon, Saskatchewan S7N 5E5, Canada

^e Institut de Chimie, UMR 7177, CNRS-Université de Strasbourg, 4 rue Blaise Pascal, Strasbourg, France

Abstract

Human cells acquire copper primarily via the copper transporter 1 protein, hCtr1. We demonstrate that at extracellular pH 7.4 Cu^II is bound to the model peptide hCtr1_1–14via an ATCUN motif and such complexes are strong enough to collect Cu^II from albumin, supporting the potential physiological role of Cu^II binding to hCtr1.

Metallomics

The N-terminal 14-mer model peptide of human Ctr1 can collect Cu(ii) from albumin. Implications for copper uptake by Ctr1†

Abstract

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