A fluorescent activatable probe for imaging intracellular Mg2+

Ryan Treadwell; Fabio de Moliner; Ramon Subiros-Funosas; Toby Hurd; Kirsten Knox; Marc Vendrell

doi:10.1039/C7OB02965A

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A fluorescent activatable probe for imaging intracellular Mg²⁺ †

Ryan Treadwell,‡^a Fabio de Moliner,‡^a Ramon Subiros-Funosas,^a Toby Hurd,^b Kirsten Knox

^c and Marc Vendrell

*^a

Author affiliations

* Corresponding authors

^a Medical Research Council Centre for Inflammation Research, The University of Edinburgh, EH16 4TJ Edinburgh, UK
E-mail: marc.vendrell@ed.ac.uk

^b MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, The University of Edinburgh, EH4 2XU Edinburgh, UK

^c Institute of Molecular Plant Sciences, The University of Edinburgh, EH9 3BF Edinburgh, UK

Abstract

An activatable BODIPY probe for in vitro detection and fluorescence cell imaging of free Mg²⁺ without interference from Ca²⁺ is described. Fluorescence amplification of the probe is observed upon detection of physiological concentrations of Mg²⁺ due to reduced rotation of the fluorophore and effective chelation by a quinolizine-based core.

This article is part of the themed collection: Chemosensors and Molecular Logic

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Article information

DOI: https://doi.org/10.1039/C7OB02965A
Article type: Paper
Submitted: 01 Dec 2017
Accepted: 12 Dec 2017
First published: 19 Dec 2017
This article is Open Access

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Org. Biomol. Chem., 2018,16, 239-244

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A fluorescent activatable probe for imaging intracellular Mg²⁺

R. Treadwell, F. de Moliner, R. Subiros-Funosas, T. Hurd, K. Knox and M. Vendrell, Org. Biomol. Chem., 2018, 16, 239 DOI: 10.1039/C7OB02965A

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Organic & Biomolecular Chemistry