Preparation and investigation of novel SrCl2/DCMC-modified (via DOPA) decellularized arteries with excellent physicochemical properties and cytocompatibility for vascular scaffolds

Abstract

A new method of fabricating vascular scaffolds was designed in this article by crosslinking the porcine arteries using dialdehyde carboxymethyl (DCMC) and further introducing the Sr element on the surface of modified arteries using DOPA. DCMC had been selected as an ideal crosslinking reagent for its excellent cytobiocompatibility and suitable chemical reactivity. Unfortunately, the endothelialization of biological vascular scaffolds fixed by DCMC was unsatisfactory. To overcome this deficiency, the Sr element was introduced onto arteries to improve the endothelialization of fixed arteries due to the Sr element being able to promote the expression of vascular endothelial growth factor (VEGF) being crucial for growth and proliferation of HUVECs. After modifying and crosslinking, their chemical structures, mechanical properties, stability, and cytocompatibility were examined. Our findings demonstrated that DCMC could improve the mechanical properties of animal-derived materials successfully and possess suitable biocompatibility compared with glutaraldehyde (GA). The Sr element can easily be introduced onto the surface of DCMC modified arteries by DOPA. Compared with purely DCMC-crosslinked ones, SrCl₂/DCMC modification has no significant effect on the mechanical strength of fixed arteries, but a slight tendancy to improve the stability of fixed samples in D-Hanks solution. MTT assay and fluorescence tests implied that SrCl₂/DCMC modification could effectively stimulate HUVECs' adhesion and proliferation, and thus promote the endothelialization process of fixed arteries. SrCl₂/DCMC-modified arteries with excellent physicochemical properties and appealing HUVEC-cytocompatibility should be promising materials for fabricating vascular scaffolds.