Complexes of magnetic nanospheres with amphiprotic polymer–Zn systems for the selective isolation of lactoferrin

Abstract

Amphiprotic polymer–Zn complex magnetic nanospheres, termed Fe₃O₄@PCL–CMC–Zn, are designed and prepared via a step-wise synthetic strategy. Hydrophobic polycaprolactone (PCL) is firstly coated onto the magnetic Fe₃O₄ nanospheres, and then hydrophilic carboxymethylcellulose (CMC) is grafted onto the hydrophobic PCL blocks via an esterification reaction, followed by finally chelating with Zn²⁺ ions. The homogeneous core–shell structure and fastened amphiprotic polymer layer provide the as-prepared Fe₃O₄@PCL–CMC–Zn magnetic nanospheres with improved protein binding behavior, and the chelated Zn²⁺ offers the nanospheres favorable adsorption selectivity towards apo-lactoferrin. The adsorption capacity of apo-lactoferrin is high, up to 615.3 mg g⁻¹. The exploitation of FeCl₃ as a stripping reagent not only provides efficient recovery of the adsorbed apo-lactoferrin, i.e. a recovery of 83.2%, but also achieves the restoration of the lactoferrin structure. The Fe₃O₄@PCL–CMC–Zn magnetic nanospheres are then employed as a sorbent for the selective isolation of lactoferrin from human colostrum samples, obtaining high-purity lactoferrin as demonstrated by SDS-PAGE and Q-TOF LC-MS assays.