NIR-Absorbing water-soluble conjugated polymer dots for photoacoustic imaging-guided photothermal/photodynamic synergetic cancer therapy

Abstract

“Theranostics” become increasingly significant in current personalized precision medicine. Herein, we developed a new NIR-absorbing photo-theranostic agent based on water-soluble diketopyrrolopyrrole (DPP) conjugated polymer (WSCP) dots. The WSCPs can be easily self-assembled into WSCP dots under ultrasonication only, instead of any other nano-technology. Compared to the monomers of WSCPs, WSCP dots have no fluorescence emission but produce photoacoustic (PA) signal detected upon laser irradiation due to the reduced energy loss from excited state. PA imaging in vivo indicated that WSCP dots can accumulate at tumor site within 4 h post-injection. More importantly, WSCP dots not only generate heat with a photothermal conversion efficiency of ∼54%, but also produce reactive oxygen species (ROS, QY ∼13%). Furthermore, in vitro and in vivo experiments confirmed effective inhibition of tumor growth by WSCP dots via synergetic photothermal/photodynamic therapy. All results indicate a great potential of WSCP dots as highly efficient theranostic agents in PA imaging-guided synergetic cancer treatment.