Issue 1, 2019

Paper-based Transwell assays: an inexpensive alternative to study cellular invasion

Abstract

Cellular movement is essential in the formation and maintenance of healthy tissues as well as in disease progression such as tumor metastasis. In this work, we describe a paper-based Transwell assay capable of quantifying cellular invasion through an extracellular matrix. The paper-based Transwell assays generate similar datasets, with equivalent reproducibility, to commercially available Transwell assays. With different culture configurations, we quantify invasion: upon addition of an exogenous factor or in the presence of medium obtained from other cell types, in an indirect or direct co-culture format whose medium composition is dynamically changing, and in a single-zone or parallel (96-zone) format.

Graphical abstract: Paper-based Transwell assays: an inexpensive alternative to study cellular invasion

Supplementary files

Article information

Article type
Paper
Submitted
23 Jun 2018
Accepted
09 Oct 2018
First published
10 Oct 2018

Analyst, 2019,144, 206-211

Paper-based Transwell assays: an inexpensive alternative to study cellular invasion

R. M. Kenney, A. Loeser, N. A. Whitman and M. R. Lockett, Analyst, 2019, 144, 206 DOI: 10.1039/C8AN01157E

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