Visual detection of cancer cells by using in situ grown functional Cu2−xSe/reduced graphene oxide hybrids acting as an efficient nanozyme

Abstract

A simple protocol for in situ growth of Cu_2−xSe nanoparticles on graphene oxide hybrids (Cu_2−xSe/rGO) acting as an efficient nanozyme is developed and thus a sensitive visual detection method of cancer cells is proposed. The Cu_2−xSe/rGO heterogeneous nanomaterials have been proven to exhibit high peroxidase-like activity to catalyze the reaction of the peroxidase substrate in the presence of hydrogen peroxide. Herein, we used Cu_2−xSe/rGO as a signal transducer to develop a colorimetric assay for the direct detection of cancer cells and a total of 63 cancer cells (MCF-7) can be distinguished by naked-eye observation. The results showed that the Cu_2−xSe/rGO hybrids could be the promising nanozyme mimetics for potential applications in bioanalytical fields.