Issue 10, 2019
From the journal:

Analyst

T7 exo-mediated FRET-breaking combined with DSN–RNAse–TdT for the detection of microRNA with ultrahigh signal-amplification†

Van Thang Nguyen,a   Binh Huy Lea  and  Young Jun Seo ORCID logo *ab  

* Corresponding authors

a Department of Bioactive Material Sciences, Chonbuk National University, South Korea

b Department of Chemistry, Chonbuk National University, South Korea
E-mail: yseo@jbnu.ac.kr
Fax: +82-63-270-3408
Tel: +82-63-270-3417

Abstract

A DSN–RNAse–TdT–T7 exo probing system allows the detection of miRNA 21 with very high sensitivity (LOD = 2.57 fM) and selectivity—the result of (i) avoiding the false-positive signal from miRNA reacting with TdT polymerase and (ii) signal amplification occurring through a FRET-breaking mechanism involving T7 exo.

Graphical abstract: T7 exo-mediated FRET-breaking combined with DSN–RNAse–TdT for the detection of microRNA with ultrahigh signal-amplification

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C9AN00303G
Article type
Communication
Submitted
14 Feb 2019
Accepted
04 Apr 2019
First published
05 Apr 2019

Analyst, 2019,144, 3216-3220

Permissions

Request permissions

T7 exo-mediated FRET-breaking combined with DSN–RNAse–TdT for the detection of microRNA with ultrahigh signal-amplification

V. T. Nguyen, B. H. Le and Y. J. Seo, Analyst, 2019, 144, 3216 DOI: 10.1039/C9AN00303G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements