Issue 42, 2019

A rapid tag-free identification of Escherichia coli antibiotic-resistant isolates using Raman scattering

Abstract

Rapid and accurate detection of pathogenic bacteria is a critical issue for public health and food safety. We introduce a rapid tag-free identification method for subtyping clinically relevant human blood-cultured Escherichia coli (E. coli) colonies. Gold-deposited surface-enhanced Raman scattering (SERS)-functionalized substrates were used to identify seven E. coli isolates. The method achieved a rapid analysis time of 30 min, an enhancement factor of 1.6 × 104 to Raman intensities, and high reproducibility with approximately 5% relative standard deviation. The proposed SERS procedure coupled with a multivariate statistics-supported classification method showed good performance compared to previous procedures. Therefore, the SERS-based tag-free identification method has high potential for rapidly subtyping clinically relevant quinolone-resistant E. coli antibiotic-resistant isolates. In future, the SERS-based biosensing technique may be applied in the clinical laboratory using a portable Raman machine and optic probe Raman system.

Graphical abstract: A rapid tag-free identification of Escherichia coli antibiotic-resistant isolates using Raman scattering

Supplementary files

Article information

Article type
Paper
Submitted
09 Aug 2019
Accepted
01 Oct 2019
First published
01 Oct 2019

Anal. Methods, 2019,11, 5381-5387

A rapid tag-free identification of Escherichia coli antibiotic-resistant isolates using Raman scattering

S. Kim, S. H. Lee, Y. J. Kim, H. J. Lee and S. Choi, Anal. Methods, 2019, 11, 5381 DOI: 10.1039/C9AY01713E

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