Issue 15, 2019
From the journal:

Chemical Communications

A semisynthetic fluorescent protein assembly-based FRET probe for real-time profiling of cell membrane protease functions in situ†

Sujuan Sun,a   Yanan Liu,a   Julan Xia,a   Miao Wang,a   Rui Tang,a   Chunyang Lei,a   Yan Huang, ORCID logo *a   Zhou Nie ORCID logo *a  and  Shouzhuo Yaoa  

* Corresponding authors

a State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha, P. R. China
E-mail: huangyan.hnu@gmail.com, niezhou.hnu@gmail.com
Fax: +86-731-88821848
Tel: +86-731-88821626

Abstract

A semisynthetic fluorescent protein assembly-based FRET probe (sFPAP) was proposed for cell membrane protease function assay. Here, the probe was anchored on a living cell membrane through a membrane inserting peptide (MIP) and was successfully utilized for in situ imaging of furin activity on the living cell membrane in real time.

Graphical abstract: A semisynthetic fluorescent protein assembly-based FRET probe for real-time profiling of cell membrane protease functions in situ

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Article information

DOI
https://doi.org/10.1039/C8CC09634A
Article type
Communication
Submitted
04 Dec 2018
Accepted
23 Jan 2019
First published
24 Jan 2019

Chem. Commun., 2019,55, 2218-2221

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A semisynthetic fluorescent protein assembly-based FRET probe for real-time profiling of cell membrane protease functions in situ

S. Sun, Y. Liu, J. Xia, M. Wang, R. Tang, C. Lei, Y. Huang, Z. Nie and S. Yao, Chem. Commun., 2019, 55, 2218 DOI: 10.1039/C8CC09634A

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