Issue 27, 2019
From the journal:

Chemical Communications

Aptamer biorecognition-triggered DNAzyme liberation and Exo III-assisted target recycling for ultrasensitive homogeneous colorimetric bioassay of kanamycin antibiotic†

Zhichao Chen,a   Feng Xiong,a   Aimin Yu ORCID logo ab  and  Guosong Lai ORCID logo *a  

* Corresponding authors

a Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, Huangshi 435002, P. R. China
E-mail: gslai@hbnu.edu.cn

b Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn VIC 3122, Australia

Abstract

A novel peroxidase-mimicking DNAzyme-based colorimetric homogenous bioassay is developed for kanamycin detection. Aptamer biorecognition triggers DNAzyme liberation from DNA duplexes for “off–on” signal transduction. The special hairpin structure of the aptamer–kanamycin biocomposite enables Exo III-assisted target recycling for signal amplification.

Graphical abstract: Aptamer biorecognition-triggered DNAzyme liberation and Exo III-assisted target recycling for ultrasensitive homogeneous colorimetric bioassay of kanamycin antibiotic

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Article information

DOI
https://doi.org/10.1039/C8CC10107H
Article type
Communication
Submitted
21 Dec 2018
Accepted
01 Mar 2019
First published
02 Mar 2019

Chem. Commun., 2019,55, 3959-3962

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Aptamer biorecognition-triggered DNAzyme liberation and Exo III-assisted target recycling for ultrasensitive homogeneous colorimetric bioassay of kanamycin antibiotic

Z. Chen, F. Xiong, A. Yu and G. Lai, Chem. Commun., 2019, 55, 3959 DOI: 10.1039/C8CC10107H

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