The lipid phase preference of the adenosine A2A receptor depends on its ligand binding state

M. Gertrude Gutierrez; Jacob Deyell; Kate L. White; Lucia C. Dalle Ore; Vadim Cherezov; Raymond C. Stevens; Noah Malmstadt

doi:10.1039/C8CC10130B

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The lipid phase preference of the adenosine A_2A receptor depends on its ligand binding state†

M. Gertrude Gutierrez,^a Jacob Deyell,^a Kate L. White,

^bcd Lucia C. Dalle Ore,

^a Vadim Cherezov,

^bcd Raymond C. Stevens

^bcd and Noah Malmstadt

*^ade

Author affiliations

* Corresponding authors

^a Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, California 90089, USA
E-mail: malmstadt@usc.edu

^b Bridge Institute, Michelson Center for Convergent Biosciences, University of Southern California, Los Angeles, CA 90089, USA

^c Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089, USA

^d Department of Chemistry, University of Southern California, Los Angeles, CA 90089, USA

^e Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USA

Abstract

Giant unilamellar protein vesicles (GUPs) were formed with the adenosine A_2A receptor (A_2AR) incorporated in the lipid bilayer and protein partitioning into the liquid ordered and liquid disordered phases was observed. When no ligand is bound, A_2AR partitions preferentially into the liquid disordered phase of GUPs, while ligand-bound A_2AR partitions into the liquid ordered phase.

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Article information

DOI: https://doi.org/10.1039/C8CC10130B
Article type: Communication
Submitted: 21 Dec 2018
Accepted: 17 Apr 2019
First published: 30 Apr 2019

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Chem. Commun., 2019,55, 5724-5727

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The lipid phase preference of the adenosine A_2A receptor depends on its ligand binding state

M. G. Gutierrez, J. Deyell, K. L. White, L. C. Dalle Ore, V. Cherezov, R. C. Stevens and N. Malmstadt, Chem. Commun., 2019, 55, 5724 DOI: 10.1039/C8CC10130B

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Chemical Communications