Issue 31, 2019

A novel fluorescent surfactant enhances the delivery of the Cas9 ribonucleoprotein and enables the identification of edited cells

Abstract

In this report, we designed and synthesized a novel fluorescent single tailed surfactant (termed FEDS), which can disrupt endosomes, complex lipofectamine, and can also identify cells that have been transfected. FEDS was able to increase the gene editing efficiency of lipofectamine/Cas9 ribonucleoprotein by 300% via a combination of fluorescent based enrichment and endosomal disruption.

Graphical abstract: A novel fluorescent surfactant enhances the delivery of the Cas9 ribonucleoprotein and enables the identification of edited cells

Supplementary files

Article information

Article type
Communication
Submitted
11 Jan 2019
Accepted
26 Mar 2019
First published
26 Mar 2019

Chem. Commun., 2019,55, 4562-4565

A novel fluorescent surfactant enhances the delivery of the Cas9 ribonucleoprotein and enables the identification of edited cells

J. Li, J. J. Røise, J. Zhang, J. Yang, D. L. Kerr, H. Han and N. Murthy, Chem. Commun., 2019, 55, 4562 DOI: 10.1039/C9CC00261H

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