Active-site directed probes based on the ubiquitin scaffold have been successfully applied as tools to determine the levels of active deubiquitinating enzymes (DUBs). Here, we show the development of a Ub-based reagent selective for the DUB USP7. This concept can be applied for the generation of other new selective reagents.
L. Gjonaj, A. Sapmaz, R. González-Prieto, A. C. O. Vertegaal, D. Flierman and H. Ovaa, Chem. Commun., 2019, 55, 5075 DOI: 10.1039/C9CC00969H
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