Synchronous screening of multiplexed biomarkers of Alzheimer's disease by a length-encoded aerolysin nanopore-integrated triple-helix molecular switch

Zhen Zou; Hua Yang; Qi Yan; Peng Qi; Zhihe Qing; Jing Zheng; Xuan Xu; Lihua Zhang; Feng Feng; Ronghua Yang

doi:10.1039/C9CC02065A

Synchronous screening of multiplexed biomarkers of Alzheimer's disease by a length-encoded aerolysin nanopore-integrated triple-helix molecular switch†

Zhen Zou,

^a Hua Yang,^a Qi Yan,^a Peng Qi,^a Zhihe Qing,

^a Jing Zheng,^b Xuan Xu,^c Lihua Zhang,^d Feng Feng

^d and Ronghua Yang

*^ab

Author affiliations

* Corresponding authors

^a Changsha University of Science and Technology, School of Chemistry and Food Engineering, Changsha, P. R. China
E-mail: Yangrh@pku.edu.cn

^b State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, P. R. China

^c Children's Medical Center, People's Hospital of Hunan Province, Changsha, Hunan 410002, P. R. China

^d College of Chemistry and Environmental Engineering, Shanxi Datong University, Datong, Shanxi, P. R. China

Abstract

Herein, we developed a versatile strategy for the synchronous screening of biomarkers of Alzheimer's disease (AD) by incorporating the high-resolution discrimination of an aerolysin nanopore to length-encoded oligonucleotides and the signal transduction of a triple-helix molecular switch (TMS). This assay is adapted to quantitatively compare multiplexed targets of very different native contents without tedious chemical labelling.

Chemical Communications

Synchronous screening of multiplexed biomarkers of Alzheimer's disease by a length-encoded aerolysin nanopore-integrated triple-helix molecular switch†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Synchronous screening of multiplexed biomarkers of Alzheimer's disease by a length-encoded aerolysin nanopore-integrated triple-helix molecular switch

Social activity

Search articles by author

Spotlight

Advertisements