Oriented attachment of VNAR proteins, via site-selective modification, on PLGA–PEG nanoparticles enhances nanoconjugate performance

João C. F. Nogueira; Michelle K. Greene; Daniel A. Richards; Alexander O. Furby; John Steven; Andrew Porter; Caroline Barelle; Christopher J. Scott; Vijay Chudasama

doi:10.1039/C9CC02655J

Oriented attachment of V_NAR proteins, via site-selective modification, on PLGA–PEG nanoparticles enhances nanoconjugate performance†

João C. F. Nogueira,^a Michelle K. Greene,^b Daniel A. Richards,^ac Alexander O. Furby,^a John Steven,^de Andrew Porter,^de Caroline Barelle,*^de Christopher J. Scott

*^b and Vijay Chudasama

*^af

Author affiliations

* Corresponding authors

^a Department of Chemistry, University College London, London, UK
E-mail: v.chudasama@ucl.ac.uk

^b Centre for Cancer Research and Cell Biology, School of Medicine, Queen's University Belfast, Belfast, UK
E-mail: c.scott@qub.ac.uk

^c Department of Materials, Imperial College London, London, UK

^d Institute of Medical Science, University of Aberdeen, Aberdeen, UK

^e Elasmogen Ltd, Aberdeen, UK
E-mail: caroline.barelle@elasmogen.com

^f Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisbon, Portugal

Abstract

Herein we report the construction of a nanoparticle-based drug delivery system which targets a key regulator in tumour angiogenesis. We exploit a Variable New Antigen Receptor (V_NAR) domain, conjugated using site-specific chemistry, to direct poly lactic acid-co-glycolic acid–polyethylene glycol (PLGA–PEG) nanoparticles to delta like canonical Notch ligand 4 (DLL4). The importance of site-specific chemistry is demonstrated.

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DOI: https://doi.org/10.1039/C9CC02655J
Article type: Communication
Submitted: 05 Apr 2019
Accepted: 29 May 2019
First published: 29 May 2019
This article is Open Access

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Chem. Commun., 2019,55, 7671-7674

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Oriented attachment of V_NAR proteins, via site-selective modification, on PLGA–PEG nanoparticles enhances nanoconjugate performance

J. C. F. Nogueira, M. K. Greene, D. A. Richards, A. O. Furby, J. Steven, A. Porter, C. Barelle, C. J. Scott and V. Chudasama, Chem. Commun., 2019, 55, 7671 DOI: 10.1039/C9CC02655J

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Chemical Communications

Oriented attachment of V_NAR proteins, via site-selective modification, on PLGA–PEG nanoparticles enhances nanoconjugate performance†

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Chemical Communications

Oriented attachment of VNAR proteins, via site-selective modification, on PLGA–PEG nanoparticles enhances nanoconjugate performance†

Abstract

Supplementary files

Article information

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Oriented attachment of VNAR proteins, via site-selective modification, on PLGA–PEG nanoparticles enhances nanoconjugate performance

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Oriented attachment of V_NAR proteins, via site-selective modification, on PLGA–PEG nanoparticles enhances nanoconjugate performance†

Oriented attachment of V_NAR proteins, via site-selective modification, on PLGA–PEG nanoparticles enhances nanoconjugate performance