A guanylurea-functionalized conjugated polymer enables RNA interference in ex vivo human airway epithelium

Md. Salauddin Ahmed; Rajib Kumar Dutta; Prakash Manandhar; Xuerong Li; Hooman Torabi; Alfonso Barrios; Ping Wang; Srinivasan Chinnapaiyan; Hoshang Jehangir Unwalla; Joong Ho Moon

doi:10.1039/C9CC02856K

A guanylurea-functionalized conjugated polymer enables RNA interference in ex vivo human airway epithelium†

Md. Salauddin Ahmed,‡^a Rajib Kumar Dutta,‡^b Prakash Manandhar,^a Xuerong Li,^a Hooman Torabi,^a Alfonso Barrios,^a Ping Wang,^c Srinivasan Chinnapaiyan,^b Hoshang Jehangir Unwalla

*^b and Joong Ho Moon

*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry and Biochemistry, Biomolecular Sciences Institute, Florida International University, 11200 SW 8th St., Miami, FL 33199, USA
E-mail: jmoon@fiu.edu

^b Department of Immunology and Nanomedicine, Florida International University, 11200 SW 8th St., Miami, FL 33199, USA

^c Department of Electric Engineering, Florida International University, 11200 SW 8th St., Miami, FL 33199, USA

Abstract

We demonstrate a successful target gene knockdown in ex vivo normal human bronchial epithelium (NHBE) cells covered with mucus layers using the guanylurea functionalization technique modulating the chemical environment at the positive charge of a gene carrier.

Chemical Communications

A guanylurea-functionalized conjugated polymer enables RNA interference in ex vivo human airway epithelium†

Abstract

Supplementary files

Article information

Download Citation

Author version available

Permissions

A guanylurea-functionalized conjugated polymer enables RNA interference in ex vivo human airway epithelium

Social activity

Search articles by author

Spotlight

Advertisements