Issue 53, 2019

A modified exponential amplification reaction (EXPAR) with an improved signal-to-noise ratio for ultrasensitive detection of polynucleotide kinase

Abstract

We applied a modified exponential amplification reaction (EXPAR) strategy to design a label-free and “one-pot” biosensor for ultrasensitive detection of polynucleotide kinase (PNK). This method was also successfully applied in the screening of PNK inhibitors and analysis of the endogenous PNK activity at the single-cell level.

Graphical abstract: A modified exponential amplification reaction (EXPAR) with an improved signal-to-noise ratio for ultrasensitive detection of polynucleotide kinase

Supplementary files

Article information

Article type
Communication
Submitted
08 May 2019
Accepted
05 Jun 2019
First published
05 Jun 2019

Chem. Commun., 2019,55, 7611-7614

A modified exponential amplification reaction (EXPAR) with an improved signal-to-noise ratio for ultrasensitive detection of polynucleotide kinase

Y. Zhang, Y. Cui, X. Li, Y. Du, A. Tang and D. Kong, Chem. Commun., 2019, 55, 7611 DOI: 10.1039/C9CC03568K

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