Visualization and quantification of cellular RNA production and degradation using a combined fluorescence and mass spectrometry characterization assay

Xiaoying Gao; Xiao Shu; Yinuo Song; Jie Cao; Minsong Gao; Fengqin Wang; Yizhen Wang; Jing Zhi Sun; Jianzhao Liu; Ben Zhong Tang

doi:10.1039/C9CC03923F

Visualization and quantification of cellular RNA production and degradation using a combined fluorescence and mass spectrometry characterization assay†

Xiaoying Gao,

‡^a Xiao Shu,‡^a Yinuo Song,^a Jie Cao,^a Minsong Gao,^a Fengqin Wang,^b Yizhen Wang,^b Jing Zhi Sun,

^a Jianzhao Liu

*^a and Ben Zhong Tang

*^cd

Author affiliations

* Corresponding authors

^a MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Zheda Road 38, Hangzhou 310027, China
E-mail: liujz@zju.edu.cn

^b College of Animal Sciences, Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Yuhangtang Road 866, Hangzhou 310058, China

^c Department of Chemistry, Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
E-mail: tangbenz@ust.hk

^d SCUT-HKUST Joint Research Laboratory, Guangdong Innovative Research Team, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou, China

Abstract

Here we report a combined fluorescence and mass spectrometry assay which is capable of stably visualizing and quantifying cellular nucleoside-labeled RNA production and degradation. The fluorescence and mass spectrometry signals from cellular labeled RNAs show a linear correlation. This simple and robust assay benefits the biological community to study RNA metabolism.

Chemical Communications

Visualization and quantification of cellular RNA production and degradation using a combined fluorescence and mass spectrometry characterization assay†

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Visualization and quantification of cellular RNA production and degradation using a combined fluorescence and mass spectrometry characterization assay

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