Issue 62, 2019
From the journal:

Chemical Communications

Chemoselective probe for detailed analysis of ketones and aldehydes produced by gut microbiota in human samples†

Louis P. Conway,a   Neeraj Garg,a   Weifeng Lin,a   Miroslav Vujasinovic,b   J.-Matthias Löhrbc  and  Daniel Globisch ORCID logo *a  

* Corresponding authors

a Department of Medicinal Chemistry, Science for Life Laboratory, Uppsala University, Box 574, SE-75123 Uppsala, Sweden
E-mail: Daniel.globisch@scilifelab.uu.se

b Department for Digestive Diseases, Karolinska University Hospital, Stockholm, Sweden

c Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institute, Stockholm, Sweden

Abstract

New strategies are required for the discovery of unknown bioactive molecules produced by gut microbiota in the human host. Herein, we utilize a chemoselective probe immobilized to magnetic beads for analysis of carbonyls in human fecal samples. We identified 112 metabolites due to femtomole analysis and an increased mass spectrometric sensitivity by up to six orders of magnitude.

Graphical abstract: Chemoselective probe for detailed analysis of ketones and aldehydes produced by gut microbiota in human samples

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Article information

DOI
https://doi.org/10.1039/C9CC04605D
Article type
Communication
Submitted
16 Jun 2019
Accepted
28 Jun 2019
First published
01 Jul 2019
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2019,55, 9080-9083

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Chemoselective probe for detailed analysis of ketones and aldehydes produced by gut microbiota in human samples

L. P. Conway, N. Garg, W. Lin, M. Vujasinovic, J.-Matthias Löhr and D. Globisch, Chem. Commun., 2019, 55, 9080 DOI: 10.1039/C9CC04605D

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