An enzyme-free DNA circuit for the amplified detection of Cd2+ based on hairpin probe-mediated toehold binding and branch migration

Jiafeng Pan; Lingwen Zeng; Junhua Chen

doi:10.1039/C9CC06311K

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An enzyme-free DNA circuit for the amplified detection of Cd²⁺ based on hairpin probe-mediated toehold binding and branch migration†

Jiafeng Pan,‡^ab Lingwen Zeng

‡^a and Junhua Chen

*^b

Author affiliations

* Corresponding authors

^a School of Food Science and Engineering, Foshan University, Foshan 528000, China

^b Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-environmental Science & Technology, Guangzhou 510650, China
E-mail: 222chenjunhua@163.com

Abstract

An enzyme-free DNA circuit was designed for the amplified detection of Cd²⁺ based on hairpin probe-mediated toehold binding and branch migration. A Cd²⁺-specific aptamer was used to recognize Cd²⁺ and a G-quadruplex was used to report the detection signal. The assay is sensitive, with a detection limit of 5 pM.

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Article information

DOI: https://doi.org/10.1039/C9CC06311K
Article type: Communication
Submitted: 14 Aug 2019
Accepted: 07 Sep 2019
First published: 09 Sep 2019

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Chem. Commun., 2019,55, 11932-11935

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An enzyme-free DNA circuit for the amplified detection of Cd²⁺ based on hairpin probe-mediated toehold binding and branch migration

J. Pan, L. Zeng and J. Chen, Chem. Commun., 2019, 55, 11932 DOI: 10.1039/C9CC06311K

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Chemical Communications